Peruzzi began his Serie A career with A.S. Roma in 1987. He was loaned to Hellas Verona in 1989, but was one of the two A.S. Roma players (the other being Andrea Carnevale) suspended for a year in October 1990 after failing a doping test because an appetite suppressant he was taking at the time contained a banned substance. His signing with Juventus in 1991 successfully revived his career, and he soon surpassed Stefano Tacconi as the Turin giants’ starting goalie. Peruzzi won three Scudetti, a UEFA Cup, and was part of Juventus’ 1996 Champions League title squad that defeated AFC Ajax in the final on penalties. After one season with Internazionale, he transferred to Lazio for €17.9 million in 2000, and made over 200 appearances in Serie A and European competition with the club.
Although his contract with the Biancocelesti ran until 2008, Peruzzi announced that he would call it quits at the end of the ’06-07 season after Lazio’s 0-0 draw with Cagliari Calcio on 15 October 2006. “I will retire at the end of the campaign,” he said. “I know the Lazio president and fans will not be happy but had I not made this decision, I would remain at the club for another six or eight years.” He later backtracked on this decision, claiming that his comments were a joke and that it was premature to talk about retirement at this early stage of the season, emphasizing that his potential retirement was being considered “one season at a time.”
After the 0-0 draw with Roma 29 April 2007, Peruzzi confirmed that he had indeed played his last match due to the frustration of nagging injuries, but he was put on for the final few minutes in Lazio’s final home match of the season, a goalless draw against Parma F.C. on 20 May as a goodbye to the fans. He was named the Serie A Goalkeeper of the Year for the third time in his career on 28 January 2008; only he and compatriot Gianluigi Buffon have won the award more than once since its inception in 1997.